CytoFLEX SRT喷嘴的清洗维护-教师体罚漫画

Cleaning and maintenance of CytoFLEX SRT nozzles

Dear CytoFLEX SRT cell sorter user,     Beckman Coulter has identified that a lack of daily and periodic cleaning and   maintenance of the CytoFLEX SRT (Product P/N: C71883, C71884, C71885, C71886,   C71887, C71888, C71889) nozzle and flow cell can result in damage to the flow   cell and/or compromise the sorting process and/or results.     This letter is to notify you of the details included in CytoFLEX SRT   Instructions For Use (IFU) regarding daily and periodic cleaning and   maintenance of the CytoFLEX SRT nozzle and flow cell.     The CytoFLEX SRT cleaning and maintenance process requires:

Regular inspection

Periodic cleaning of the nozzle

Replacement of the nozzle’s O-ring when        damaged, or once a month during systematic use as indicated in the CytoFLEX        SRT IFU

When inspecting the nozzle and O-ring, it   is recommended to use a microscope with 40X magnification to inspect the   nozzle for salt and/or other debris and examine the O-ring for damage.   Representative images of good and damaged O-rings are shown below in Figures   1 and 2, respectively:

Figure 2: Representative images of damaged O-rings and dirty nozzles under magnification

All the details to clean the CytoFLEX SRT   nozzle tip are described in the CytoFLEX SRT IFU chapter “Cleaning   Procedures” under “Cleaning the Nozzle” section.     When the nozzle O-ring needs to be replaced due to age or damage, it is VERY   IMPORTANT for system stability that the O-ring is installed in the   proper orientation as detailed in the IFU chapter “Replacement/Adjustment   Procedures” under “Nonscheduled Replacement/Adjustment”, Section “Replacing   the O-ring.” Note that the O-ring has a narrow portion that faces up and a   wider portion that faces down when installed. Please reference the drawing   below in Figure 3 for guidance.

Figure 3: CytoFLEX SRT nozzle diagram showing proper       O-ring orientation for installation             Finally, it is very important to NOT remove the       nozzle when the system is initialized (i.e. when the sheath stream is       running). Removing the nozzle holder when the stream is on may result in       damage to optical components in the flow cell and, as a consequence, a       degradation of detection might occur. Following       the daily cleaning procedures detailed in the IFU chapter “Shutting Down       the System” will also help maintain the full sensitivity and function of       the instrument. You can find the latest version of the CytoFLEX SRT IFU       at www.beckman.com for full details about the above procedures or other       cleaning and maintenance topics.             Please share this information with your laboratory staff as necessary. If       you have any questions regarding this notice, please contact our Customer       Support Center at 1-800-369-0333 from the United States or Canada.       Outside of US and Canada, contact your local Beckman Coulter       Representative.             Thank you,